Kerrie Dolick had a hat trick with two assists from Abby Wagner as the Muskies moved to 4-2 in the MVL and 5-4 overall.
Wagner scored off a Jacqueline Williams assist at the 24:11 mark of the first half, then found Dolick for her first goal about three minutes later to stake John Glenn to a 2-0 halftime lead.
Dolick scored twice — the second assisted by Wagern — in the final nine minutes of the game.
John Glenn had 15 shots to six by Maysville. Hannah Stephen had six saves in the win. Shawna Lambert had eight saves and Karsyn Shatto three for the Panthers (1-5).
Mackenzie Prater and Holly Jones had seven steals each for John Glenn.
John Glenn reserves tied River View 0-0.